This Mouse Bmi1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptid
e between 109-143 amino acids from the Central region of Mouse Bmi1.
背景介紹
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcription
ally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remod
eling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably
changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of
RNF2/RING2 (By similarity).
All lanes : Anti-Bmi1 Antibody (Center) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse kidney lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band
Anti-Bmi1 Antibody (Center)at 1:2000 dilution + mouse heart lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.